An easy-to-produce non-integrating option for gene expression

With virtually no limits on insert size (unlike AAV vectors) Enhanced Episomal Vectors (EEVs) are an excellent choice for non-integrating, non-viral gene expression. Because they replicate in synchrony with the host cell, they are stably inherited and can be used for long-lasting expression—up to several months both in vitro and in vivo—without modifying the host genome. Use SBI’s CAGs-MCS EEV Vector (Cat.# EEV600A-1) for constitutive expression in most cell types including primary cells and stem cells with the strong CAG promoter.

SBI’s EEV System, which is an enhanced version of the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA1) system, offers:

• High levels of expression
• Easy to clone formats
• No special plasmid production
• Nonviral, non-integrating technology
• Constitutive and inducible vector formats

Please note the following Licensing Restrictions for the EEV system:

For Academic and Non-Profit Institutions:

Researchers at academic and non-profit institutes are granted full access to purchasing the EEV cloning vectors and reporters. Full sequence information is provided upon proof of purchase of EEV vectors. Please contact SBI’s technical support at tech@systembio.com for sequence information.

For Commercial Customers:

For-profit and commercial customers can purchase the pre-made EEV reporters (EEV604A-1, EEV605A-1); however, due to licensing restrictions, cloning EEV vectors (e.g. EEV600A-1 and EEV610A-1) are not available for purchase directly. Instead, these are available through SBI’s custom EEV cloning and production services. SBI then provides the commercial customer with the desired amount of ready-to-use EEV custom construct DNA.

美国SBI代理 System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1